Proteomics Services

Identification

• Identification/simple sample analysis ESI-QqTof MS/MS (BrukerMicrOTOFQ) MASCOT
• Identification Protein /simple sample analysis MALDI MS/MS (BrukerMicrOTOFQ)

Protein identification in complex samples (Shotgun proteomics)

• Identification in complex nano HPLC samples ESI-QqTof MS/MS (BrukerMicrOTOFQ) MASCOT
• Identification by De Novo sequencing HPLC ESI-QqTof MS/MS

Discovery peptide profiles

• LC/MS profile nanoHPLC sample ESI-QqTof MS (Agilent 1200 BrukerMicrOTOFQ) (ProfileAnalysis)
• Clinical proteomic MS ClinProt profiles and statistics (ClinProtTools)

Targeted peptide profiles

• LC/MS profile nanoHPLC sample ESI-QqTof MS (Agilent 1200 BrukerMicrOTOFQ)
• uPLC ESI-QQQ Analysis, MRM (Agilent 1260 Agilent 6410BA)

Absolute and relative quantification

• Isotopic labelling
• Label free
• Post-translation modification analysis
• Characterization of immunoprecipitated proteins
• Purification of recombinant proteins and antibodies using low pressure chromatography
• FPLC Äktabasic with combined column, affinity, high-performance exclusion, ion-exchange and reverse phase strategies
• Characterization of intact human recombinant proteins T3 MS
• MALDI Imaging

Metabolomics Services

Profiles of metabolites of all types (volatile, semi-volatile and non-volatile, both polar and non-polar) generally for biomarker discovery 

• GC/MS Analysis (Thermo GC8060 MD800 EI+, CI-, CI+)
• LC/MS profile nanoHPLC sample ESI-QqTof MS (Agilent 1200 BrukerMicrOTOFQ) (ProfileAnalysis)
• uPLC ESI-QQQ Analysis (Agilent 1260 Agilent 6410BA)

Identification and description of metabolites

• GC/MS analysis (Thermo GC8060 MD800 EI+)
• LC/MS/MS analysis nanoHPLC sample ESI-QqTof MS
• Absolute and relative quantification of small molecules
• GC/MS SIM analysis (Thermo GC8060 MD800 EI+, CI-, CI+)
• GC FID analysis (Perkin Elmer)
• LC/MS profile nanoHPLC sample ESI-QqTof MS (Agilent 1200 BrukerMicrOTOFQ) (ProfileAnalysis)
• uPLC analysis ESI-QQQ , MRM (Agilent 1260 Agilent 6410BA)

Within this section our unit has developed procedures for:

• medium and large short-chain fatty acids in serum and cell membranes
• amino acids
• organic acids in urine
• acylcarnitines in plasma
• prostanoids in plasma
• volatile carbon compounds in breath
• free plasmatic thyroid hormones
• steroid hormones in saliva
• sugars in urine
• mucopolysaccharides in serum and urine